Coding

Part:BBa_K4131002

Designed by: Chun Ying Yu   Group: iGEM22_CCU_Taiwan   (2022-10-09)


AccE gene (C. glutamicum), codon optimized for E. Coli expression

The acetyl-CoA carboxylase of C. glutamicum is composed of a biotin carboxylase (AccBC), a biotin carboxyl carrier protein (AccE), and a transcarboxylase (AccD1). They carboxylated acetyl-CoA into malonyl-CoA, which is necessary for the elongation of nascent fatty acid. The carboxylation occurs in two steps. In the first step, the ATP-dependent biotin carboxylase (AccBC) catalyzes the transfer of a carboxyl group to biotin, which is linked to the biotin carboxyl carrier protein (AccE). In the second step, the transcarboxylase (AccD1) transferred the carboxyl group from carboxybiotin to acetyl-CoA to form malonyl-CoA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None